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This lecture is partially adapted from the IMISS1 course `` Atmospheric Absorption'' By Michel Guélin (sections 10.1 and 10.4.1). It also includes a description of the phase correction methods (sections 10.5.3 and 10.5.4) by Martina Wiedner.The author would like also to acknowledge the contributions of the following people: J. Cernicharo, E. Serabyn and S. Matsushita.